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Electrophysiological experiment : in 73 rats , extracellular recordings in vivo were made from pvn using 3 - barrel microelectrode . neurons were categorized as gastric distension excitatory ( gd - e ) or inhibitory ( gd - i ) neurons tested with gastric distension stimulus . drugs were applied through the 3 - barrel microelectrode by a 4 - programmable pressure injector ( pm2000b , mdi , usa ) : relin , saline ( control group ) relin , " [ d - lys - 3 ] - ghrp - 6 ( antagonist for ghrelin - r ) , to observe the effects of drugs on the neuron discharge
電生理實(shí)驗(yàn)方法：在73只大鼠中，應(yīng)用三管玻璃微電極細(xì)胞外記錄麻醉大鼠一側(cè)pvn神經(jīng)元自發(fā)放電，用水囊充盈胃鑒別胃擴(kuò)張敏感神經(jīng)元( gdsn ) ，以壓力注射儀( pm2000b ， mdi ， usa )經(jīng)三管玻璃微電極，對(duì)核團(tuán)內(nèi)中文摘要單個(gè)神經(jīng)元分別微量注射給予: ghrelin 、生理鹽水ns (對(duì)照) ghrelin 、 d一lys一3 ]一ghrp一6 ( ghrelin受體拮抗劑) ，觀察藥物對(duì)神經(jīng)元單位放電的影響。